6 beta-hydroxycortisol interferes with immunoassay of urinary free cortisol
نویسندگان
چکیده
منابع مشابه
6 beta-hydroxycortisol in serum and urine as determined by enzyme immunoassay on microtitre plates.
We modified our previous enzyme immunoassay (Clin Chim Acta 1986;157:267-76) for estimating 6 beta-hydroxycortisol (6 beta-OHF) to provide a more routine procedure for measuring it in urine and to increase sensitivity for its measurement in serum. Precision, analytical recovery, specificity, and detection limit are reported. 6 beta-OHF and 17-hydroxycorticosteroids in 24-h and 4-h (08:00-12:00 ...
متن کاملUrinary 6 beta-hydroxycortisol/cortisol ratios measured by high-performance liquid chromatography for use as a biomarker for the human cytochrome P-450 3A4.
Cortisol is metabolized to 6 beta-hydroxycortisol by the human cytochrome P-450 3A4, an enzyme implicated in the critical epoxidation reactions of aflatoxin B1 and certain polycyclic aromatic hydrocarbons. A method has been developed to measure the 6 beta-hydroxycortisol:cortisol ratio in human urine by reverse-phase high-performance liquid chromatography using diode-array detection. This metho...
متن کاملAssociation between urinary 6β-hydroxycortisol/cortisol ratio and CYP3A5 genotypes in a normotensive population
Genetic polymorphism of genes involved in renal salt handling and arterial vessel tone is considered to be one of the causes of hypertension. Numerous reports suggest that cytochrome P4503A5 (CYP3A5) catalyzes 6β-hydroxylation of endogenous cortisol (CS), which is associated with sodium and water retention in the kidney and involved in the regulation of blood pressure. The purpose of the presen...
متن کاملFluorometric measurement of urinary free hydrocortisone (cortisol).
Submitters: Charles R. Ratliff and Frank F. Hall, Section of Biochemistr’.,’, Scott & White Clinic; and Scott & White Hospital Foundation, Temple, Tex. 76501 Evaluators: Thomas W. CuIp, BioRegional Reference Laboratory, Medical Arts Building, San Antonio, Tex. 78205 Christopher S. Frings, Medical Laboratory Associates, 1Q25 South 18th St., Birmingham. Ala. 3520.5 James L. Gilleland and John E. ...
متن کاملDetermination of urinary free cortisol by HPLC.
We here report a reversed-phase HPLC method for the determination of free cortisol in human urine, using methylprednisolone as the internal standard. Before chromatography, samples were extracted with a C18 solid-phase extraction column and the steroids were separated on a LiChrospher 100 C18 column with a mobile phase of methanol/acetonitrile/water (43/3/54 by vol). Linearity, precision, and a...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 1996
ISSN: 0009-9147,1530-8561
DOI: 10.1093/clinchem/42.8.1290